Journal: Scientific reports

Article Title: Apatinib weakens proliferation, migration, invasion, and angiogenesis of thyroid cancer cells through downregulating pyruvate kinase M2.

doi: 10.1038/s41598-023-50369-w

Figure Lengend Snippet: Figure 1. Apatinib markedly restrained proliferation migration, and invasion of SW579 and TPC-1 cells. (A) CCK8 was used to measure the cell proliferation of SW579 and TPC-1 cells. *P < 0.05, **P < 0.01, vs. Blank. (B) EdU staining was used to detect cell proliferation of of SW579 and TPC-1 cells. (C) Wound healing demonstrated the change of cell migration ability in Apatinib-treated SW579 and TPC-1 cells. Magnification, 100 ×. (D) Cell invasion was evaluated through Transwell assay in SW579 and TPC-1 cells after processing with Apatinib. (E) Western blot was utilized to analyze the change of N-cadherin, Vimentin, E-cadherin expressions in SW579 and TPC-1 cells after administration with Apatinib. Magnification, 200 ×.

Article Snippet: SW579 and TPC-1 cells were first disposed of 20 ng/ml, 50 ng/ml, 100 ng/ml Apatinib (Selleck Chemicals; cat. no. S5248) for 48 h51.

Techniques: Migration, Staining, Transwell Assay, Western Blot

Journal: Scientific reports

Article Title: Apatinib weakens proliferation, migration, invasion, and angiogenesis of thyroid cancer cells through downregulating pyruvate kinase M2.

doi: 10.1038/s41598-023-50369-w

Figure Lengend Snippet: Figure 2. Apatinib prominently suppressed HUVEC tube formation and weakened PKM2 expression in TC. (A) Images of tube formation assay in HUVECs, which were incubated with the conditioned medium from Apatinib-treated SW579 and TPC-1 cells. Magnification, 100 ×. (B) After incubation with the conditioned medium, IF staining was conducted to assess the expression change of VEGFR2 in HUVECs. Magnification, 200 ×. (C) The expression change of VEGFA was credited through IF staining in HUVECs after treatment with the conditioned medium from Apatinib-processed SW579 and TPC-1 cells. Magnification, 200 ×. (D) Western blot was utilized to analyze the change of VEGFA and PKM2 expressions in SW579 and TPC-1 cells after administration with Apatinib.

Article Snippet: SW579 and TPC-1 cells were first disposed of 20 ng/ml, 50 ng/ml, 100 ng/ml Apatinib (Selleck Chemicals; cat. no. S5248) for 48 h51.

Techniques: Expressing, Tube Formation Assay, Incubation, Staining, Western Blot

Journal: Scientific reports

Article Title: Apatinib weakens proliferation, migration, invasion, and angiogenesis of thyroid cancer cells through downregulating pyruvate kinase M2.

doi: 10.1038/s41598-023-50369-w

Figure Lengend Snippet: Figure 3. PKM2 overexpression notably reversed Apatinib-mediated suppression of proliferation, migration, and invasion in SW579 and TPC-1 cells. (A) SW579 and TPC-1 cells were treated with Apatinib or/and PKM2 overexpression plasmid, EdU staining was conducted to examine the change in cell proliferative activity. Magnification, 200 ×. (B) CCK8 was used to measure the cell proliferation of SW579 and TPC-1 cells. **P<0.01 VS. Control. #P<0.05 vs. 50ng/mL + vector. (C) Cell migration was credited via wound healing. Magnification, 100 ×. (D) Transwell presented the change in cell invasion capacity in each group. (E) Western blot displayed the change levels of N-cadherin, Vimentin, E-cadherin expressions in SW579 and TPC-1 cells. Magnification, 200 ×.

Article Snippet: SW579 and TPC-1 cells were first disposed of 20 ng/ml, 50 ng/ml, 100 ng/ml Apatinib (Selleck Chemicals; cat. no. S5248) for 48 h51.

Techniques: Over Expression, Migration, Plasmid Preparation, Staining, Activity Assay, Control, Western Blot

Journal: Scientific reports

Article Title: Apatinib weakens proliferation, migration, invasion, and angiogenesis of thyroid cancer cells through downregulating pyruvate kinase M2.

doi: 10.1038/s41598-023-50369-w

Figure Lengend Snippet: Figure 4. PKM2 overexpression dramatically attenuated the inhibitory effect of Apatinib on HUVEC tube formation in SW579 and TPC-1 cells. (A) Apatinib-processed SW579 and TPC-1 cells were then transfected with PKM2 overexpression plasmid. After incubation with the conditioned medium from the treated SW579 and TPC-1 cells, the angiogenesis was assessed by applying tube formation assay in HUVECs. Magnification, 100 ×. (B) The change in VEGFR2 expression was examined through IF staining in the processed HUVECs. Magnification, 200 ×. (C) IF staining was adopted to evaluate the change of VEGFA expression in each group. Magnification, 200 ×. (D) Western blot displayed the change levels of PKM2 and VEGFA expressions in SW579 and TPC-1 cells.

Article Snippet: SW579 and TPC-1 cells were first disposed of 20 ng/ml, 50 ng/ml, 100 ng/ml Apatinib (Selleck Chemicals; cat. no. S5248) for 48 h51.

Techniques: Over Expression, Transfection, Plasmid Preparation, Incubation, Tube Formation Assay, Expressing, Staining, Western Blot